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Protocol
Springer Protocols
Quantification of Human Chemokine Production in TLR-Stimulated and Antigen-Specific Recall Responses
Chemokines are primarily low molecular mass proteins that are produced and usually released by a wide variety of cell types. Differential chemokine responses can be excellent early markers of immune dysfunction, allowing clinical intervention prior
… moreTechniques: Immunoassay, ELISA, Titration, Cell And Tissue Culture, Data analysis
Models: Homo sapiens
Antibodies: Anti-CD4, Anti-CD80, Anti-CD86, Anti-human IP-10, Anti-CD3... 2 more
Citations: 6 | Downloads: 3,28710.1007/978-1-59745-366-0_11 -
Protocol
Springer Protocols
Screening for Compounds That Affect the Interaction Between Bacterial Two-Component Signal Transduction Response Regulator Protein and Cognate Promoter DNA
Bacterial signal transduction systems can be used as drug targets. The signal transduction targets fall into two groups–-sensor kinases and response regulators. Previously reported studies describe hits that were thought to inactivate sensor kinases
… moreModels: Escherichia coli BL21, Pseudomonas aeruginosa
Downloads: 2,03310.1007/978-1-59745-246-5_17 -
Protocol
Springer Protocols
Computer-Assisted Reading of DNA Sequences
DNA sequencing is increasingly used in a range of medical activities involving DNA diagnostics and research. This is the result of improving technology and cheaper costs. Paradoxically, a greater demand for DNA sequencing has placed additional work
… moreTechniques: DNA Sequencing, Base-calling, PCR, Sequence Analysis, Sequence Comparison... 3 more
Citations: 4 | Downloads: 1,11710.1007/978-1-60327-148-6_10 -
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Protocol
Springer Protocols
Short-Term Culture of CD8 Cells and Intracellular Cytokine Staining
CD8 T cells play an important role in the regulation of allergic disease. Human and murine CD8 T cells have been shown to be capable of differentiating into distinct subsets defined by cytokine profiles analogous to the Th1 and Th2 subsets and termed
… moreTechniques: Intracellular Cytokine Staining, Flow Cytometry, Density-gradient Centrifugation, Cell And Tissue Culture, Cell Fixation
Models: Homo sapiens
Antibodies: Anti-CD3, Anti-CD28, Anti-IL-4, Anti-IL-12
Downloads: 3,62810.1007/978-1-59745-366-0_7 -
Protocol
Springer Protocols
Conjugation of Haptens
Many naturally occurring proteins, peptides, carbohydrates, nucleic acids, and lipids, as well as synthetic peptides, are successful immunogens. To elicit an immune response, a compound must contain an antigenic determinant or epitope and must be of
… moreTechniques: Immunoassay, Cross-linking, Dialysis, Rearrangement, Gel Electrophoresis... 6 more
Models: Homo sapiens, Bos taurus
Citations: 15 | Downloads: 4,23810.1007/978-1-59745-366-0_14 -
Overview
Springer Protocols
Utilizing HapMap and Tagging SNPs
Advancements in our understanding of variation in the human genome and rapid improvements in high-throughput genotyping technology have made it feasible to study most of the human genetic diversity that is due to common variations in relation to
… moreTechniques: Genotyping
Citations: 9 | Downloads: 1,21710.1007/978-1-60327-148-6_3 -
Protocol
Springer Protocols
Protein Comparative Sequence Analysis and Computer Modeling
A problem frequently encountered by the biological scientist is the identification of a previously unknown gene or protein sequence, where there are few or no clues as to the biochemical function, ligand specificity, gene regulation, protein–protein
… moreTechniques: Protein Structure Prediction, Protein Secondary Structure, Protein Function Prediction, Sequence Analysis, Structure Prediction... 12 more
Downloads: 1,11410.1007/978-1-60327-148-6_13 -
Protocol
Springer Protocols
Collection of Air Samples to Quantify Exposure to Airborne Allergens
Under normal conditions, airborne allergens are present at very low concentrations. Allergens may be carried on relatively large identifiable particles such as grains of pollen and mould spores or smaller amorphous particles or both. The methods that
… moreTechniques: Immunoassay
Citations: 3 | Downloads: 3,28310.1007/978-1-59745-366-0_17 -
Overview
Springer Protocols
Clinical Uses of Microarrays in Cancer Research
Perturbations in genes play a key role in the pathogenesis of cancer. Microarray-based technology is an ideal way in which to study the effects and interactions of multiple genes in cancer. There are many technologic challenges in running a
… moreTechniques: Microarray
Citations: 12 | Downloads: 1,19010.1007/978-1-60327-148-6_6 -
Protocol
Springer Protocols
Online Resources for the Molecular Contextualization of Disease
Searching online resources can provide medical researchers with an efficient means of gathering existing knowledge on the molecular causes of disease. The researcher may choose to explore the following areas, e.g., genetic mutations associated with
… moreTechniques: Cross-linking, Protein Sequence Analysis, Image Analysis, Recombinant Protein Expression, Yeast Two-hybrid Assay... 4 more
Downloads: 1,09810.1007/978-1-60327-148-6_16